Applications and advantages of the use of cell cultures
What is a cell culture?
Types of cell cultures
cell lines
Evolution of cell culture (cell culture surfaces and media)
Necessary conditions for cell culture
Containers and substrates
Culture media
Types of growing medium
Supplements
Environmental conditions
aseptic technique
Pollution
Growth phases of a cell culture
Subcultures
Monitoring
cell count
Cell cryopreservation
Storage
Practical session
Preparation of solutions for cell culture
Sample Thawing
cell seeding
Cell culture monitoring
Principles
System Components
MicroBeads
Separator
Columns
Purification Types
Positive selection
Negative selection
Product portfolio
Automated equipment
Clinical grade products
Sample Preparation o
Practical session
Purification of a specific population from peripheral blood by magnetic separation
Components of a flow cytometer
The Fluidics system
The Optical configuration
The Electronic system
Data Analysis
Antibodies and fluorochromes
Design of an experiment
Practical session
Analysis of a peripheral blood sample with the MACSQuant flow cytometer